Chapter 12. The Nervous System and Nervous Tissue

12.5 The Action Potential

Learning Objectives

By the end of this section, you will be able to:

Describe how movement of ions across the neuron membrane leads to an action potential

  • Describe the components of the membrane that establish the resting membrane potential
  • Describe the changes that occur to the membrane that result in the action potential

The functions of the nervous system—sensation, integration, and response—depend on the functions of the neurons underlying these pathways. To understand how neurons are able to communicate, it is necessary to describe the role of an excitable membrane in generating these signals. The basis of this process is the action potential. An action potential is a predictable change in membrane potential that occurs due to the open and closing of voltage gated ion channels on the cell membrane.

Electrically Active Cell Membranes

Most cells in the body make use of charged particles (ions) to create electrochemical charge across the cell membrane. In a prior chapter, we described how muscle cells contract based on the movement of ions across the cell membrane. For skeletal muscles to contract, due to excitation–contraction coupling, they require input from a neuron. Both muscle and nerve cells make use of a cell membrane that is specialized for signal conduction to regulate ion movement between the extracellular fluid and cytosol.

As you learned in the chapter on cells, the cell membrane is primarily responsible for regulating what can cross the membrane. The cell membrane is a phospholipid bilayer, so only substances that can pass directly through the hydrophobic core can diffuse through unaided. Charged particles, which are hydrophilic, cannot pass through the cell membrane without assistance (Figure 12.51). Specific transmembrane channel proteins permit charged ions to move across the membrane. Several passive transport channels, as well as active transport pumps, are necessary to generate a transmembrane potential, and an action potential. Of special interest is the carrier protein referred to as the sodium/potassium pump that uses energy to move sodium ions (Na+) out of a cell and potassium ions (K+) into a cell, thus regulating ion concentration on both sides of the cell membrane.

This diagram shows a cross section of a cell membrane. The cell membrane proteins are large, blocky, objects. Peripheral proteins are not embedded in the phospholipid bilayer. The peripheral protein shown here is attached to the outside surface of another protein on the extracellular fluid side. Integral proteins are embedded between the phospholipids of the cell membrane. The transmembrane integral protein extends through both phospholipids layers. The opposite ends of this protein project into the cytosol and the extracellular fluid. A second, smaller integral protein only extends into the inner phospholipid layer. Its opposite end projects into the cytosol. This second protein is, therefore, not a transmembrane protein. The channel protein is cylinder shaped with a hollow internal tube labeled the pore. The sides of the channel protein can bulge inward to close the pore.

Figure 12.51 – Cell Membrane and Transmembrane Proteins: The cell membrane is composed of a phospholipid bilayer and has many transmembrane proteins, including different types of channel proteins that serve as ion channels.

The sodium/potassium pump requires energy in the form of adenosine triphosphate (ATP), so it is also referred to as an ATPase pump. As was explained in the cell chapter, the concentration of Na+ is higher outside the cell than inside, and the concentration of K+ is higher inside the cell than outside. Therefore, this pump is working against the concentration gradients for sodium and potassium ions, which is why it requires energy. The Na+/K+ ATPase pump maintains these important ion concentration gradients.

Ion channels are pores that allow specific charged particles to cross the membrane in response to an existing electrochemical gradient. Proteins are capable of spanning the cell membrane, including its hydrophobic core, and can interact with charged ions because of the varied properties of amino acids found within specific regions of the protein channel. Hydrophobic amino acids are found in the regions that are adjacent to the hydrocarbon tails of the phospholipids, where as hydrophilic amino acids are exposed to the fluid environments of the extracellular fluid and cytosol. Additionally, ions will interact with the hydrophilic amino acids, which will be selective for the charge of the ion. Channels for cations (positive ions) will have negatively charged side chains in the pore. Channels for anions (negative ions) will have positively charged side chains in the pore. The diameter of the channel’s pore also impacts the specific ions that can pass through.  Some ion channels are selective for charge but not necessarily for size. These nonspecific channels allow cations—particularly Na+, K+, and Ca2+—to cross the membrane, but exclude anions.

Some ion channels do not allow ions to freely diffuse across the membrane, but are gated instead. A ligand-gated channel opens because a molecule, or ligand, binds to the extracellular region of the channel (Figure 12.52).

These two diagrams each show a channel protein embedded in the cell membrane. In the left diagram, there is a large number of sodium ions (NA plus) and calcium ions (CA two plus) in the extracellular fluid. Within the cytosol, there is a large number of potassium ions (K plus) but only a few sodium ions. In this diagram, the channel is closed. Two ACH molecules are floating in the extracellular fluid. Their label indicates that a neurotransmitter, a ligand, is required to open the ion channel. The neurotransmitter receptor site on the extracellular fluid side of the channel protein matches the shape of the ACH molecules. In the right diagram, the two ACH molecules attach to the neurotransmitter receptor sites on the channel protein. This opens the channel and the sodium and calcium ions diffuse through the channel and into the cytosol, down their concentration gradient. The potassium ions also diffuse through the channel in the opposite direction down their concentration gradient (out of the cell and into the extracellular fluid).

Figure 12.52 – Ligand-Gated Channels: When the ligand, in this case the neurotransmitter acetylcholine, binds to a specific location on the extracellular surface of the channel protein, the pore opens to allow select ions through. The ions, in this case, are cations of sodium, calcium, and potassium.

A mechanically-gated channel opens because of a physical distortion of the cell membrane. Many channels associated with the sense of touch are mechanically-gated. For example, as pressure is applied to the skin, mechanically-gated channels on the subcutaneous receptors open and allow ions to enter (Figure 12.53).

These two diagrams each show a channel protein embedded in the cell membrane. In the left diagram, there are a large number of sodium ions in the extracellular fluid, but only a few sodium ions in the cytosol. There is a large number of calcium ions in the cytosol but only a few calcium ions in the extracellular fluid. In this diagram, the channel is closed, as the extracellular side has a lid, somewhat resembling that on a trash can, that is closed over the channel opening. In the right diagram, the mechanically gated channel is open. This allows the sodium ions to flow from the extracellular fluid into the cell, down their concentration gradient. At the same time, the calcium ions are moving from the cytosol into the extracellular fluid, down their concentration gradient.

Figure 12.53 – Mechanically-Gated Channels: When a mechanical change occurs in the surrounding tissue (such as pressure or stretch) the channel is physically opened, and ions can move through the channel, down their concentration gradient.

A voltage-gated channel is a channel that responds to changes in the electrical properties of the membrane in which it is embedded. Normally, the inner portion of the membrane is at a negative voltage. When that voltage becomes less negative and reaches a value specific to the channel, it opens and allows ions to cross the membrane (Figure 12.54).

Figure 12.54 – Voltage-Gated Channels: Voltage-gated channels open when the transmembrane voltage changes around them. Amino acids in the structure of the protein are sensitive to charge and cause the pore to open to the selected ion.

A leak channel is randomly gated, meaning that it opens and closes at random, hence the reference to leaking. There is no actual event that opens the channel; instead, it has an intrinsic rate of switching between the open and closed states. Leak channels contribute to the resting transmembrane voltage of the excitable membrane (Figure 12.55).

This is a two part diagram. Both diagrams show a leakage channel embedded in the lipid membrane bilayer. The leakage channel is cylindrical with a large, central opening. In the first diagram there are several ions in the cytosol but only one ion in the extracellular fluid. No ions are moving through the leakage channel because the channel is closed. In the second diagram, the leakage channel randomly opens, allowing two ions to travel through the channel, down their concentration gradient, and out into the extracellular fluid.

Figure 12.55 – Leak Channels: These channels open and close at random, allowing ions to pass through when they are open.

The Membrane Potential

The membrane potential is a distribution of charge across the cell membrane, measured in millivolts (mV). The standard is to compare the inside of the cell relative to the outside, so the membrane potential is a value representing the charge on the intracellular side of the membrane (based on the outside being zero, relatively speaking; Figure 12.56).

This diagram shows a cross section of a cell membrane. The extracellular fluid side of the cell membrane is positively charged while the cytosol side of the membrane is negatively charged. There is a microelectrode embedded in the cell membrane. The microelectrode is attached to a voltmeter, which also has a reference electrode on the extracellular fluid side. The readout of the voltmeter is negative 70 millivolts.

Figure 12.56 – Measuring Charge across a Membrane with a Voltmeter: A recording electrode is inserted into the cell and a reference electrode is outside the cell. By comparing the charge measured by these two electrodes, the transmembrane voltage is determined. It is conventional to express that value for the cytosol relative to the outside.

There is typically an overall net neutral charge between the extracellular and intracellular environments of the neuron. However, a slight difference in charge occurs right at the membrane surface, both internally and externally. It is the difference in this very limited region that holds the power to generate electrical signals, including action potentials, in neurons and muscle cells.

When the cell is at rest, ions are distributed across the membrane in a very predictable way. The concentration of Na+ outside the cell is 10 times greater than the concentration inside. Also, the concentration of K+ inside the cell is greater than outside. The cytosol contains a high concentration of anions, in the form of phosphate ions and negatively charged proteins. With the ions distributed across the membrane at these concentrations, the difference in charge is described as the resting membrane potential. The exact value measured for the resting membrane potential varies between cells, but -70 mV is a commonly reported value. This voltage would actually be much lower except for the contributions of some important proteins in the membrane. Leak channels allow Na+ to slowly move into the cell or K+ to slowly move out, and the Na+/K+ pump restores their concentration gradients across the membrane. This may appear to be a waste of energy, but each has a role in maintaining the membrane potential.

The Action Potential

Resting membrane potential describes the steady state of the cell, which is a dynamic process balancing ions leaking down their concentration gradient and ions being pumped back up their concentration gradient. Without any outside influence, the resting membrane potential will be maintained. To get an electrical signal started, the membrane potential has to become more positive.

This starts with the opening of voltage-gated Na+ channels in the neuron membrane. Because the concentration of Na+ is higher outside the cell than inside the cell by a factor of 10, ions will rush into the cell, driven by both the chemical and electrical gradients. Because sodium is a positively charged ion, as it enters the cell it will change the relative voltage immediately inside the cell membrane. The resting membrane potential is approximately -70 mV, so the sodium cation entering the cell will cause the membrane to become less negative. This is known as depolarization, meaning the membrane potential moves toward zero (becomes less polarized). The concentration gradient for Na+ is so strong that it will continue to enter the cell even after the membrane potential has become zero, so that the voltage immediately around the pore then begins to become positive.

As the membrane potential reaches +30 mV, slower to open voltage-gated potassium channels are now opening in the membrane. An electrochemical gradient acts on K+, as well. As K+ starts to leave the cell, taking a positive charge with it, the membrane potential begins to move back toward its resting voltage. This is called repolarization, meaning that the membrane voltage moves back toward the -70 mV value of the resting membrane potential.

Repolarization returns the membrane potential to the -70 mV value of the resting potential, but overshoots that value. Potassium ions reach equilibrium when the membrane voltage is below -70 mV, so a period of hyperpolarization occurs while the K+ channels are open. Those K+ channels are slightly delayed in closing, accounting for this short overshoot.

What has been described here is the action potential, which is presented as a graph of voltage over time in Figure 12.57. It is the electrical signal that nervous tissue generates for communication. The change in the membrane voltage from -70 mV at rest to +30 mV at the end of depolarization is a 100-mV change.

This graph has membrane potential, in millivolts, on the X axis, ranging from negative 70 to positive thirty. Time is on the X axis. The plot line starts steadily at negative seventy and then increases to negative 55 millivolts. The plot line then increases quickly, peaking at positive thirty. This is the depolarization phase. The plot line then quickly drops back to negative seventy millivolts. This is the repolarization phase. The plot line continues to drop but then gradually increases back to negative seventy millivolts. The area where the plot line is below negative seventy millivolts is the hyperpolarization phase.

Figure 12.57 – Graph of Action Potential: Plotting voltage measured across the cell membrane against time, the action potential begins with depolarization, followed by repolarization, which goes past the resting potential into hyperpolarization, and finally the membrane returns to rest.

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What happens across the membrane of an electrically active cell is a dynamic process that is hard to visualize with static images or through text descriptions. View this animation to learn more about this process. What is the difference between the driving force for Na+ and K+? And what is similar about the movement of these two ions?

The membrane potential will stay at the resting voltage until something changes. To begin an action potential, the membrane potential must change from the resting potential of approximately -70mV to the threshold voltage of -55mV. Once the cell reaches threshold, voltage-gated sodium channels open and being the predictable membrane potential changes describe above as an action potential.  Any sub-threshold depolarization that does not change the membrane potential to -55 mV or higher will not reach threshold and thus will not result in an action potential. Also, any stimulus that depolarizes the membrane to -55 mV or beyond will cause a large number of channels to open and an action potential will be initiated.

Because of the predictable changes that occur once threshold is reached, the action potential is referred to as “all or none”. This means that either the action potential occurs and is repeated along the entire length of the neuron or no action potential occurs. A stronger stimulus, which might depolarize the membrane well past threshold, will not make a “bigger” action potential. Either the membrane reaches the threshold and everything occurs as described above, or the membrane does not reach the threshold and nothing else happens. All action potentials peak at the same voltage (+30 mV), so one action potential is not bigger than another. Stronger stimuli will initiate multiple action potentials more quickly, but the individual signals are not bigger.

As we have seen, the depolarization and repolarization of an action potential are dependent on two types of channels (the voltage-gated Na+ channel and the voltage-gated K+ channel). The voltage-gated Na+ channel actually has two gates. One is the activation gate, which opens when the membrane potential crosses -55 mV. The other gate is the inactivation gate, which closes after a specific period of time—on the order of a fraction of a millisecond. When a cell is at rest, the activation gate is closed and the inactivation gate is open. However, when the threshold is reached, the activation gate opens, allowing Na+ to rush into the cell. Timed with the peak of depolarization, the inactivation gate closes. During repolarization, no more sodium can enter the cell. When the membrane potential passes -55 mV again, the activation gate closes. After that, the inactivation gate re-opens, making the channel ready to start the whole process over again.

The voltage-gated K+ channel has only one gate, which is sensitive to a membrane voltage of -50 mV. However, it does not open as quickly as the voltage-gated Na+ channel does. It takes a fraction of a millisecond for the K+ channel to open once that voltage has been reached, which coincides exactly with when the Na+ flow peaks. So voltage-gated K+ channels open just as the voltage-gated Na+ channels are being inactivated. As the membrane potential repolarizes and the voltage passes -50 mV again, the K+ channels begin to close. Potassium continues to leave the cell for a short while and the membrane potential becomes more negative, resulting in the hyperpolarization overshoot. Then the K+ channels are closed and the membrane returns to the resting potential because of the ongoing activity of the leak channels and the Na+/K+ ATPase pump.

All of this takes place within approximately 2 milliseconds (Figure 12.58). While an action potential is in progress, another one cannot be initiated. That effect is referred to as the refractory period. There are two phases of the refractory period: the absolute refractory period and the relative refractory period. During the absolute refractory period, another action potential will not start. This is because of the inactivation gate of the voltage-gated Na+ channel. Once the Na+ channel is back to its resting conformation, a new action potential could be started during the hyperpolarization phase, but only by a stronger stimulus than the one that initiated the current action potential.

This graph has membrane potential, in millivolts, on the X axis, ranging from negative 70 to positive thirty. Time is on the X axis. In step one, which is labeled at rest, the plot line is steady at negative seventy millivolts. In step 2, a stimulus is applied, causing the plot line to increase to positive 30 millivolts. The curve sharply increases at step three, labeled voltage rises. After peaking at positive thirty, the plot line then quickly drops back to negative 70. This is the fourth step, labeled voltage falls. The plot line continues to drop below negative 70 and this is step 5, labeled end of action potential. Finally, the plot line gradually increases back to negative seventy millivolts, which is step 6, labeled return to rest.

Figure 12.58 – Stages of an Action Potential: Plotting voltage measured across the cell membrane against time, the events of the action potential can be related to specific changes in the membrane voltage. (1) At rest, the membrane voltage is -70 mV. (2) The membrane begins to depolarize when an external stimulus is applied. (3) The membrane voltage begins a rapid rise toward +30 mV. (4) The membrane voltage starts to return to a negative value. (5) Repolarization continues past the resting membrane voltage, resulting in hyperpolarization. (6) The membrane voltage returns to the resting value shortly after hyperpolarization.

Propagation of the Action Potential

The action potential is initiated at the beginning of the axon, at what is called the initial segment (trigger zone). Rapid depolarization can take place here due to a high density of voltage-gated Na+ channels. Going down the length of the axon, the action potential is propagated because more voltage-gated Na+ channels are opened as the depolarization spreads. This spreading occurs because Na+ enters through the channel and moves along the inside of the cell membrane. As the Na+ moves, or flows, a short distance along the cell membrane, its positive charge depolarizes a little more of the cell membrane. As that depolarization spreads, new voltage-gated Na+ channels open and more ions rush into the cell, spreading the depolarization a little farther.

Because voltage-gated Na+ channels are inactivated at the peak of the depolarization, they cannot be opened again for a brief time (absolute refractory period). Because of this, positive ions spreading back toward previously opened channels has no effect. The action potential must propagate from the trigger zone toward the axon terminals.

Propagation, as described above, applies to unmyelinated axons. When myelination is present, the action potential propagates differently, and is optimized for the speed of signal conduction. Sodium ions that enter the cell at the trigger zone start to spread along the length of the axon segment, but there are no voltage-gated Na+ channels until the first node of Ranvier. Because there is not constant opening of these channels along the axon segment, the depolarization spreads at an optimal speed. The distance between nodes is the optimal distance to keep the membrane still depolarized above threshold at the next node. As Na+ spreads along the inside of the membrane of the axon segment, the charge starts to dissipate. If the node were any farther down the axon, that depolarization would have fallen off too much for voltage-gated Na+ channels to be activated at the next node of Ranvier. If the nodes were any closer together, the speed of propagation would be slower.

Propagation along an unmyelinated axon is referred to as continuous conduction; along the length of a myelinated axon it is referred to as saltatory conduction. Continuous conduction is slow because there are always voltage-gated Na+ channels opening, and more and more Na+ is rushing into the cell. Saltatory conduction is faster because the action potential “jumps” from one node to the next (saltare = “to leap”), and the new influx of Na+ renews the depolarized membrane. Along with the myelination of the axon, the diameter of the axon can influence the speed of conduction. Much as water runs faster in a wide river than in a narrow creek, Na+-based depolarization spreads faster down a wide axon than down a narrow one. This concept is known as resistance and is generally true for electrical wires or plumbing, just as it is true for axons, although the specific conditions are different at the scales of electrons or ions versus water in a river.

Homeostatic Imbalances – Potassium Concentration
Glial cells, especially astrocytes, are responsible for maintaining the chemical environment of the CNS tissue. The concentrations of ions in the extracellular fluid are the basis for how the membrane potential is established and changes in electrochemical signaling. If the balance of ions is upset, drastic outcomes are possible.

Normally the concentration of K+ is higher inside the neuron than outside. After the repolarizing phase of the action potential, K+ leak channels and Na+/K+ pumps ensure that the ions return to their original locations. Following a stroke or other ischemic event, extracellular K+ levels are elevated. The astrocytes in the area are equipped to clear excess K+ to aid the pump. But when the level is far out of balance, the effects can be irreversible.

Astrocytes can become reactive in cases such as these, which impairs their ability to maintain the local chemical environment. The glial cells enlarge and their processes swell. They lose their K+ buffering ability and the function of the pump is affected, or even reversed. One of the early signs of cell disease is this “leaking” of sodium ions into the body cells. This sodium/potassium imbalance negatively affects the internal chemistry of cells, preventing them from functioning normally.

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Visit this site to see a virtual neurophysiology lab, and to observe electrophysiological processes in the nervous system, where scientists directly measure the electrical signals produced by neurons. Often, the action potentials occur so rapidly that watching a screen to see them occur is not helpful. A speaker is powered by the signals recorded from a neuron and it “pops” each time the neuron fires an action potential. These action potentials are firing so fast that it sounds like static on the radio. Electrophysiologists can recognize the patterns within that static to understand what is happening. Why is the leech model used for measuring the electrical activity of neurons instead of using humans?

 

 

Chapter Review

The nervous system is characterized by electrical signals that are sent from one area to another. Whether those areas are close or very far apart, the signal must travel along an axon. The basis of the electrical signal is the controlled distribution of ions across the membrane. Transmembrane ion channels regulate when ions can move in or out of the cell, so that a precise signal is generated. This signal is the action potential which has a very characteristic shape based on voltage changes across the membrane in a given time period.

The membrane is normally at rest with established Na+ and K+ concentrations on either side. A stimulus will start the depolarization of the membrane, and voltage-gated channels will result in further depolarization followed by repolarization of the membrane. A slight overshoot of hyperpolarization marks the end of the action potential. While an action potential is in progress, another cannot be generated under the same conditions. While the voltage-gated Na+ channel is inactivated, absolutely no action potentials can be generated. Once that channel has returned to its resting state, a new action potential is possible, but it must be started by a relatively stronger stimulus to overcome the state of hyperpolarization.

The action potential travels down the axon as voltage-gated ion channels are opened by the spreading depolarization. In unmyelinated axons, this happens in a continuous fashion because there are voltage-gated channels throughout the membrane. In myelinated axons, propagation is described as saltatory because voltage-gated channels are only found at the nodes of Ranvier and the electrical events seem to “jump” from one node to the next. Saltatory conduction is faster than continuous conduction, meaning that myelinated axons propagate their signals faster. The diameter of the axon also makes a difference as ions diffusing within the cell have less resistance in a wider space.

 

Interactive Link Questions

What happens across the membrane of an electrically active cell is a dynamic process that is hard to visualize with static images or through text descriptions. View this animation to really understand the process. What is the difference between the driving force for Na+ and K+? And what is similar about the movement of these two ions?

Sodium is moving into the cell because of the immense concentration gradient, whereas potassium is moving out because of the depolarization that sodium causes. However, they both move down their respective gradients, toward equilibrium.

Visit this site to see a virtual neurophysiology lab, and to observe electrophysiological processes in the nervous system, where scientists directly measure the electrical signals produced by neurons. Often, the action potentials occur so rapidly that watching a screen to see them occur is not helpful. A speaker is powered by the signals recorded from a neuron and it “pops” each time the neuron fires an action potential. These action potentials are firing so fast that it sounds like static on the radio. Electrophysiologists can recognize the patterns within that static to understand what is happening. Why is the leech model used for measuring the electrical activity of neurons instead of using humans?

The properties of electrophysiology are common to all animals, so using the leech is an easier approach to studying the properties of these cells. There are differences between the nervous systems of invertebrates (such as a leech) and vertebrates, but not for the sake of what these experiments study.

 

Review Questions

Critical Thinking Questions

1. What does it mean for an action potential to be an “all or none” event?

2. The conscious perception of pain is often delayed because of the time it takes for the sensations to reach the cerebral cortex. Why would this be the case based on propagation of the axon potential?

Glossary

absolute refractory period
time during an action period when another action potential cannot be generated because the voltage-gated Na+ channel is inactivated
activation gate
part of the voltage-gated Na+ channel that opens when the membrane voltage reaches threshold
continuous conduction
slow propagation of an action potential along an unmyelinated axon owing to voltage-gated Na+ channels located along the entire length of the cell membrane
depolarization
change in a cell membrane potential from rest toward zero
electrochemical exclusion
principle of selectively allowing ions through a channel on the basis of their charge
excitable membrane
cell membrane that regulates the movement of ions so that an electrical signal can be generated
gated
property of a channel that determines how it opens under specific conditions, such as voltage change or physical deformation
inactivation gate
part of a voltage-gated Na+ channel that closes when the membrane potential reaches +30 mV
ionotropic receptor
neurotransmitter receptor that acts as an ion channel gate, and opens by the binding of the neurotransmitter
leakage channel
ion channel that opens randomly and is not gated to a specific event, also known as a non-gated channel
ligand-gated channels
another name for an ionotropic receptor for which a neurotransmitter is the ligand
mechanically gated channel
ion channel that opens when a physical event directly affects the structure of the protein
membrane potential
distribution of charge across the cell membrane, based on the charges of ions
nonspecific channel
channel that is not specific to one ion over another, such as a nonspecific cation channel that allows any positively charged ion across the membrane
refractory period
time after the initiation of an action potential when another action potential cannot be generated
relative refractory period
time during the refractory period when a new action potential can only be initiated by a stronger stimulus than the current action potential because voltage-gated K+ channels are not closed
repolarization
return of the membrane potential to its normally negative voltage at the end of the action potential
resistance
property of an axon that relates to the ability of particles to diffuse through the cytoplasm; this is inversely proportional to the fiber diameter
resting membrane potential
the difference in voltage measured across a cell membrane under steady-state conditions, typically -70 mV
saltatory conduction
quick propagation of the action potential along a myelinated axon owing to voltage-gated Na+ channels being present only at the nodes of Ranvier
size exclusion
principle of selectively allowing ions through a channel on the basis of their relative size
voltage-gated channel
ion channel that opens because of a change in the charge distributed across the membrane where it is located

Solutions

Answers for Critical Thinking Questions

  1. The cell membrane must reach threshold before voltage-gated Na+ channels open. If threshold is not reached, those channels do not open, and the depolarizing phase of the action potential does not occur, the cell membrane will just go back to its resting state.
  2. Axons of pain sensing sensory neurons are thin and unmyelinated so that it takes longer for that sensation to reach the brain than other sensations.